... tissuelyser (Tissuelyser LT, Quiagen) to break the cell aggregates. To collect the single cells, the sample was pulse-centrifuged in a tabletop microcentrifuge. The supernatant was diluted 1:2 with reactor medium until an OD600 of 0.45 ...
... Serial 1:10 dilutions were spotted (5 μl) onto plates containing the ... AutoCAD 2010 (Autodesk, Inc., San Rafael, CA, United States). For this ... number of vesicles present along at least 68 fields of vision per biological replicate ...
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