author: erica a golemis

... NaHPO4<!> (pH 7.2), this is 0.25 M Na2HPO4 with the pH adjusted to 7.2 with ortho-phosphoric acid (see Orlando et al. 1997) Wash buffer 5% SDS<!> 1 mM EDTA 0.5% BSA 40 mM NaHPO4<!> (pH 7.2) Cells Chosen tissue or cell line; in this case ...